Extraction of Lepidium sativum Seed Mucilage: Optimization of Extraction Process with Maximum Yield by using Full Factorial Design

Objective: The present investigation helps to isolate the seed mucilage of Lepidium sativum by using an economic extraction process with utmost yield. Diligence effort was given to get the maximum yield of the mucilage with good flow properties without affecting the intrinsic properties of the extracted mucilage. The yield of the seed mucilage of Lepidium sativum was optimized by using a quality by design approach. The full factorial design was used using three variables (pH of extract, water to seed ratio, and drying temperature) at two levels (high and low). Overall, ten formulation trials were generated through statistical software Minitab placing 2 center points, 1 replicate, and 1 block. All trials were executed to evaluate the percentage yield.

Results: The extraction process was evaluated with different solvents (such as ethanol and acetone) and different techniques to monitor the yield of the process. The maximum yield was achieved by soaking Lepidium sativum seeds in water (1:30 ratio), maintaining an alkaline pH, blending by hand blender, and using acetone as a solvent. A factorial design was used to observe the responses like the yield of the isolated mucilage for different levels of all the independent variables. A polynomial equation was developed and model plots (contour plot and Pareto chart) were generated to study the impact of the critical variables on the response yield. ANOVA analysis of the model suggested that the independent variables had significantly affected (p < 0.05) in predicting the response (% yield) and the coefficient terms with a p-value less than 0.05 had a significant effect on the prediction efficacy of the model. Finally, the optimized parameter was established based on statistical evaluation obtained from the polynomial equation as well as the recommendation obtained from the response optimizer. The study indicates that the obtained linear regression equation has a linear relation with a regression coefficient of 0.9869 % for response % yield.

Conclusion: A cost-effective extraction and isolation process was established to extract the seed mucilage of Lepidium sativum with maximum yield. The isolated mucilage was evaluated for flow properties and demonstrated that it is having good flow properties and can be used as an excipient or adjuvant in different pharmaceuticals and food industries. The applicability of factorial design was well demonstrated to optimize the yield by optimizing the critical factors.