In Vitro Shoot Multiplication in Ocimum basilicum L.

Profuse callus initiation was obtained from internode and leaf explants inoculated on MS medium supplemented with 1 mg/l 2,4-D. Indirect organogenesis was observed from the leaf and internode explants inoculated on MS medium supplemented with 0.3-0.5 mg/l IAA and 3-5 mg/l BA. Multiple shoots were initiated from the shoot tip and nodal explants inoculated on MS medium fortified with BA or Kin alone or in combination. Maximum multiple shoots were obtained in MS medium supplemented with 5 mg/l BA and 10 mg/l Kin. The shoots were obtained via direct and indirect organogenesis were further sub cultured on MS medium supplemented with 4mg/l BA and 1mg/l GA3 Numerous shoots were obtained after repeated subcultures. Rooting was observed from the shoot in the regeneration medium. The plantlets on hardening showed 85% survival rate.