Estimation of Chlorophyll Pigment in Cyamopsis tetragonoloba (Cluster bean) in Different Soil Samples

Present study was carried out in Cyamopsis tetragonoloba plant leaves grown in various soil samples and calculated values of Chlorophyll a and chlorophyll b, Total chlorophyll from leaves of Cyamopsis tetragonoloba. A green pigment called chlorophyll can be found in juvenile branches and leaves of plants and trees. The amount of leaf chlorophyll and the type of photosynthetic mechanism present, which in turn affect crop production and yield, are crucial parameters to measure. The synthesis of chlorophyll needs through a series of enzymatic reactions, with the temperatures that are too high or low inhibiting the enzyme reaction, even destroying the original chlorophyll. It was found that total chlorophyll concentration is high in cluster bean or Cyamopsis tetragonoloba grown in agriculture soil and low in deforested soil. Chlorophyll a:b ratio is used to characterize physiological state of plants under water and salinity stress conditions. Chlorophyll a:b ratio is high for Cyamopsis tetragonoloba plant grown in deforested soil and low for agriculture soil.