A Study on the Evaluation of Biomedical Activity of ZnO Coated Bionanocomposites Film

Biopolymer-based ZnO nanocomposite film was synthesized using cellulose as a supporting agent and the extract of Livistona jekinsiana as a reducing agent. Chitosan and b-cyclodextrin were used in the synthesis of bionanocomposites film to utilize the antibacterial property and superior delivery property respectively. Objectives of our work are to synthesize a biodegradable, biocompatible, and economically sound new and novel cellulose/chitosan/b-cyclodextrin/ Livistona jekinsiana/ZnO bionanocomposites film (CCCLZF) via green methods and used as a promising biomedical applicant for the first time. UV-visible spectrum at 352 nm revealed the synthesis of ZnO NPs outside the film in the plant extracts. X-Ray Diffraction pattern of CCCLZF showed characteristic peaks at 2 = 32. , 35. , 36. , 7. , 56. , 62. and 67. corresponding to the planes (1 0 0), (0 0 2), (1 0 1), (1 0 2), (1 1 0), (1 0 3), and (1 1 2) that revealed the close-packed hexagonal Wurtzite structure of ZnO NPs. The incorporation of NPs into the biopolymer matrix was ascertained by the intense peak around 20. (0 0 2). FTIR spectrum of CCCLZF showed bands around 3399.76 and 2919.64 cm-1 due to OH and CH2 asymmetric stretching of biopolymer. The absorption band at 578.78 cm-1 corresponded to the Zn-O bind in the LJ-ZnO NPs. SEM and TEM images of CCCLZF determined the uniform, spherical shape and uniform distribution of the LJ-ZnO NPs. SEM images showed that there were no severe agglomerations of ZnO NPs in the biopolymer matrix which helped in the uniform distribution of the LJ-ZnO NPs. EDS analysis of the CCCLZF confirmed the presence of C, O, and Zn in the film. TGA and DTA curves of the CCCLZF exhibited 4% weight loss at C and thermal decomposition at C, respectively. The average value of the zeta potential of ZnO NPs was 3.34 mV with a standard deviation of 1.23 which revealed the stability of NPs with low agglomeration. CCCLZF inhibited the growth of Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, and one fungal strain Candida albicans at lower MIC 20 g/ml, 35 g/ml, 25 g/ml, 35 g/ml, and 25 g/ml respectively. Antioxidant activity and anti-inflammatory activity with IC50 values at 124.45 g/ml and 96.78 g/ml were determined for CCCLZF film. Drug loading capacity and encapsulation efficiency of CCCLZF film were 34.50% and 29.78%. Therefore, CCCLZF will be a promising material in the pharmaceutical and food packaging industry.